> For the complete documentation index, see [llms.txt](https://mysite.gitbook.io/sdas_manual_cn/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://mysite.gitbook.io/sdas_manual_cn/readme/04_manual/tf/02_input.md).

# 输入文件示例

## h5ad数据格式说明

* `test.h5ad`：空间转录组表达矩阵AnnData对象，要求包含**原始表达矩阵**、**细胞类型注释**等信息。具体请参考[SDAS输入数据格式描述](/sdas_manual_cn/readme/04_manual/sdas_input.md)。

## celltype\_list细胞类型列表文件（非必须）

* `celltype_list.txt:`自定义细胞类型列表文件，无表头，每行一个细胞类型

<table data-header-hidden><thead><tr><th width="114.54541015625"></th></tr></thead><tbody><tr><td>Fibroblast</td></tr><tr><td>T/NK</td></tr></tbody></table>

## &#x20;TF\_list转录因子列表文件（非必须）

* `TF_list.txt:`自定义转录因子列表文件，无表头，每行一个转录因子

<table data-header-hidden><thead><tr><th width="114.54541015625"></th></tr></thead><tbody><tr><td>CLOCK(+)</td></tr><tr><td>E2F3(+)</td></tr><tr><td>EBF1(+)</td></tr><tr><td>E1S1(+)</td></tr></tbody></table>

## 转录因子数据库说明

* **内置数据库来源**：
  * 转录因子列表（TF list）：包含目标物种的所有已知转录因子基因名<https://github.com/aertslab/pySCENIC/tree/master/resources>
  * 参考数据库（Feather文件）：存储基因与转录因子结合位点（motif）的调控潜能排序。<https://resources.aertslab.org/cistarget/databases/>
  * 转录因子注释文件：关联 motif 与对应转录因子的映射关系。<https://resources.aertslab.org/cistarget/motif2tf/>
* **自定义数据库构建可参考：**
  * pySCENIC官方文档：[**https://pyscenic.readthedocs.io/en/latest/**](https://pyscenic.readthedocs.io/en/latest/)
  * CistTarget数据库说明：[**https://resources.aertslab.org/cistarget/**](https://resources.aertslab.org/cistarget/)


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