Input File Example

h5ad Data Format Description

• st.h5ad: Spatiotemporal transcriptomics expression matrix AnnData object, which must include the raw expression matrix, gene names, and differential analysis grouping information. For details, please refer to SDAS input data format description

• When using DESeq2 or edgeR for differential analysis with biological replicates, the raw expression matrix must be used.

• Avoid special characters such as spaces, /, +, ,, ~, etc. in the keys and values of the h5ad file, otherwise the process may report errors