Performance Test

Stereo-seq Test Data

Sample
Cell Number
Cell Types (n)

sample1_bin100

17,884

29

sample1_bin50

70,761

38

sample1_bin20

439,383

39

sample1_cellbin

134,703

39

sample2_bin100

23,061

11

sample2_bin50

91,106

11

sample2_bin20

5,643,322

11

Runtime and Memory Usage

  • Squidpy: Both runtime and memory usage increase with the number of cells. For different bin sizes, the runtime is typically less than 1 hour. However, memory usage increases significantly for bin20.

  • CRAWDAD: Runtime and memory usage also increase with the number of cells. For different bin sizes, memory usage generally remains below 10 GB, but runtime increases significantly for bin20.

  • CRAWDAD: The runtime is highly sensitive to the value of --crawdad_perms. Increasing this parameter improves the accuracy of p-values but also substantially increases computation time. The default value is 10.

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