Input File Example

h5ad Data Format Description

• st.h5ad: The AnnData object of the spatial transcriptomics expression matrix, which must contain raw expression matrix and spatial coordinates information. For details, please refer to SDAS Input Data Format Description.

Moran Index File Format Description (Optional)

• ./moran.csv: Precomputed gene Moran index file (including results of squidpy Moran's I index analysis, the first two columns are required: gene name consistent with h5ad and moranI), separated by commas. Example content is as follows:

	moranI	pval_norm	var_norm	pval_norm_fdr_bh	real_gene_name
ENSG00000164433	0.8273024881077159	0.0	0.0	0.0001022749701474042	FABP5
ENSG00000106078	0.8203151436536835	0.0	0.0	0.0001022749701474042	STX3
ENSG00000170260	0.7993499975159156	0.0	0.0	0.0001022749701474042	HSPB1
ENSG00000163220	0.797949719212986	0.0	0.0	0.0001022749701474042	S100A9
ENSG00000187021	0.7868677451739036	0.0	0.0	0.0001022749701474042	NTS