Performance Test

Stereo-seq Test Data

We tested a total of 11 samples, including mouse brain and various human tumor samples. Each sample was tested with cellbin, bin20, bin50, and bin100 (if available). The number of genes in bin20 data ranges from about 100 to 900.

Cellbin
Bin20
Bin50
Bin100

Number of genes

300~1,000

100-900

600-4,000

1,900+

Number of cells

300,000-400,000

500,000-700,000

80,000-120,000

20,000-30,000

Running Time and Memory Usage

  • The NeST/hotspot/hdWGCNA algorithms use n_cpus to control the number of parallel threads. This performance test uses n_cpus=8.

  • Running time and memory requirements are positively correlated with the number of cells and genes, i.e., the larger the data scale, the higher the computation time and memory usage.

  • Running time: hotspot > nest/hdWGCNA

  • Memory: nest/hdWGCNA > hotspot

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